Table 1 Immunohistochemical methods used in this study.
Antibody (Clone/Product code) | Dilution | Immunostaining method | Source of primary antibody |
|---|---|---|---|
CK5/6 (D5/16 B4) | 1:200 | Dako Envision Kit | DakoCytomation, Glostrup, Denmark |
EGFR (NCL-L-EGFR-384) | 1:200 | Dako Envision Kit | Leica Biosystems, Newcastle, United Kingdom |
ER (NCL-ER-6F11) | 1:50 | Dako Envision Kit | Novocastra, Newcastle upon Tyne, United Kingdom |
HER2 (NCL-CB11) | 1:500 | Dako Envision Kit | Novocastra |
PR (PgR 636) | 1:150 | Dako Envision Kit | DakoCytomation |
PRDX III (LF-P A 0030) | 1:500 | Histostain-Plus Bulk Kit | Labfrontier, York, United Kingdom |
PRDX IV (LF-P A 0009) | 1:500 | Histostain-Plus Bulk Kit | Labfrontier |
8-OHdG (N45.1) | 1:50 | Dako Envision Kit | JaICA, Fukuroi, Japan |
Nrf2 (SC-722) | 1:100 | Histostain Plus Broad Spectrum Kit | Santa Cruz Biotechnology, Santa Cruz, USA |
Keap1 (SC-15246) | 1:100 | Biocare Medical HRP Polymer Kit | Santa Cruz |