Figure 1

RKIP and pRKIP protein expression lung cancer cell lines. Different lung cancer cells lines were grown in RPMI with 10% of FBS. Total protein was extracted from the culture and then separated by SDS-PAGE and transferred onto the nitrocellulose membrane as described in Material and methods. The membrane was stained with anti-RKIP or anti p-RKIP. (lane 1) A549 cell line, (lane 2) H157 cell line, (lane 3) BEA52B cell line. The β-actin antibody was used as a loading control. The findings revealed that the differentially pRKIP expression between the different lung cell lines analyzed.