Figure 4

Cladribine induces activation of caspase-3, -8, -9 and PARP cleavage in MM cells. A, U266 cells were untreated or treated with indicated concentrations of cladribine for 24 hrs. B & C, RPMI8226 or MM1.S cells were untreated or treated with cladribine (1 μmol/L or 0.2 μmol/L, respectively) for 16, 24, or 48 hrs. Cells were collected and subjected to western blot analyses with specific antibodies directed against caspase-3 (Casp-3), caspase-8 (Casp-8), caspase-9 (Casp-9), PARP, or β-actin.