Figure 4

Down-regulation of NDRG2 increases the adhesion, migration and invasion of Huh7 cells. NDRG2-high HCC Huh7 cells were transfected with NDRG2 siRNA (siRNA-N2) or the negative control siRNA (siRNA-NC) using Lipofectamine 2000. The cells were subjected to adhesion (A and B), migration (C and D) and invasion assays (E and F), respectively. In (B), (D) and (F), the histograms represent the quantification of cells attached, wounding remaining width and cells having invaded through Matrigel. Data represent three independent experiments. *P < 0.05 between groups.