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Figure 3 | BMC Cancer

Figure 3

From: Deletion of the thrombin cleavage domain of osteopontin mediates breast cancer cell adhesion, proteolytic activity, tumorgenicity, and metastasis

Figure 3

uPA expression and proteolytic activity of 468-CON, 468-OPN, and 468-ΔTC cells. (A)Quantitative RT-PCR of uPA mRNA levels collected from cells grown on 3 separate 60 mm dishes over 3 experiments. Expression was adjusted to GAPDH internal control. (B)Representative gel of zymographic analysis. (C)Conditioned media equivalent to 5 × 103 cells was subjected to zymographic analysis and density of bands was measured using BioRad Universal Hood II and Quantity One 4.6.1 software (BioRad). Experiments were replicated 3 times. Data represents % control of 468-CON cells and is presented as the mean +/- SEM. * = sig. different than corresponding treatment of 468-CON (p < 0.01); δ = sig. different than corresponding treatment of 468-OPN (p < 0.001).

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BMC Cancer

ISSN: 1471-2407

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