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Figure 2 | BMC Cancer

Figure 2

From: c-Myc activates BRCA1 gene expression through distal promoter elements in breast cancer cells

Figure 2

Activation of BRCA1 expression by c-Myc in breast cancer cells. A vector of overexpression c-Myc (pMyc-GFP) and the reporter/luciferase constructs were co-transfected into MCF-7 or MDA-MB-231 cells as describer in methods. The amounts of Myc-GFP expression vectors are as indicated. (A) The constitutively expressed Myc-GFP fusion protein (Myc-GFP) and endogenous c-Myc (c-Myc) from the same amounts of whole cellular protein extracts from each cell line are detected by western blot. Actins are used as the loading control (Actin). (B) BRCA1 promoter activities from whole cell lysates were measured. The luciferase data were normalized to the empty vector and compare to that without overexpression of c-Myc as 1 (column: 0 ng). Bars show mean ± SD. *: p < 0.01 as each indicated column compared to the 0 ng column.

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