Figure 1

Reduction of expressions of BRCA1 mRNA and BRCA1 protein, and BRCA1 promoter activity by depletion of c-Myc in breast cancer cells. (A) MCF-7 or MDA-MB-231 cells were treated with c-Myc siRNA to deplete c-Myc expression, and total cellular c-Myc and BRCA1 protein levels were determined by western blot with actin levels serving as a protein loading control. (B) BRCA1 mRNA expression levels from cells treated with c-Myc siRNA were determined by quantitative RT-PCR. Real-time PCR was performed in two independent experiments in triplicate. Bars show mean ± SE. *: p < 0.01. (C) The BRCA1 promoter reporter vector pCYL42 was transfected into cells with depleted c-Myc by c-Myc siRNA treatments, and BRCA1 promoter activities were recorded. The data were presented as means of control siRNA as 1, bars show mean ± SD. *: p < 0.01.