Skip to main content
Figure 7 | BMC Cancer

Figure 7

From: FAP-overexpressing fibroblasts produce an extracellular matrix that enhances invasive velocity and directionality of pancreatic cancer cells

Figure 7

FAP + matrix-induced Panc-1 invasion is dependent of FAK. Panc-1 cells were cultured within the assorted matrices for 2 days in the presence of mAb13, ATN-161, or rabbit sera. The specific activity of the kinases was calculated as the ratio of the scanned optical densities of the phosphorylated forms divided by total expression levels (P/T). The fold of P/T ratios being assessed obtained from FAP- matrices (square box) was assigned as one arbitrary unit. (A) Constitutive FAK activity levels of Panc-1 cells induced by FAP+ matrix were up-regulated by ~1.5 fold (p = 0.007). Inhibition of β1-integrin activity significantly blocked the FAK activity levels of Panc-1 cells cultured within both FAP- and FAP+ matrices (p = 0.02 and p = 0.002, respectively). However, inhibition of α5β1-integrin showed no significant changes in FAK activity levels (all p > 0.3). (B) AKT activity in Panc-1 cells on matrices remained unchanged in the presence or absence of inhibitory agents. Statistical significances were marked using asterisks.

Back to article page