+ ECMs promote a tumor permissive microenvironment. To test the effects of FAP on the behavior of cancer cells, Panc-1 cells on assorted ECMs were monitored using time-lapse acquisition. Compared to their behavior within FAP- matrix, Panc-1 cells presented increased velocity and directionality within FAP+ matrix (p = 0.05 and p < 0.001, respectively). When compared to behaviors induced by FAP+ matrices, Panc-1 cells on FAP+inhibitor matrix attenuated net path distances (D) and directionality (p = 0.07 and p = 0.01, respectively), but not velocity (p = 0.19). Statistical significances were marked using asterisks.