Figure 3

H-318, ST-486 and LS-123 cell lines TP53 sequencing analysis. (A) H-318 mouse adenocarcinoma cell line TP53 sequencing. The mutated nucleotide (TP53 exon 5, codon 172, CGC > CAC, Arg > His) is marked with an arrow. Only the mutated DNA is detected, suggesting a hemi/homozygous mutation. (B) ST-486 cell line sequencing. 1. DNA analysis showed a heterozygous mutation in codon 158 (TP53 exon 5, CGC > CAC, Arg > His), both mutated and wild-type nucleotides are detected. 2. Exon 7 sequencing showed the second - heterozygous mutation (TP53, codon 239, AAC > GAC, Asn > Asp), both nucleotides are visible. (C) LS-123 cell line DNA sequencing, the mutated nucleotide (TP53 exon 5, codon 175, CGC > CAC, Arg > His) is marked with an arrow. The analysis shows undetectable wild type DNA, suggesting homo/hemizygous mutation. Mutated nucleotides are marked with arrows. N - normal, control template.