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Figure 1 | BMC Cancer

Figure 1

From: Detection of antibodies directed at M. hyorhinis p37 in the serum of men with newly diagnosed prostate cancer

Figure 1

Purification of recombinant M. hyorhinis p37 protein by affinity chromotography. M. hyorhinis p37 (MH38-113) was expressed in E. coli and purified as described previously [16]. Sonicated cell lysate of E. coli was applied to a cobalt affinity column, and the bound protein was eluted with 150 mM imidazole. A total of 25 μg eluate was electrophoresed in a 12% SDS-PAGE gel, and stained with Coomassie blue. M, Prestained BenchMark Protein ladder (kDa); 1, purified recombinant protein. The arrow indicates the position of the recombinant protein.

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