Figure 1From: Detection of antibodies directed at M. hyorhinis p37 in the serum of men with newly diagnosed prostate cancer Purification of recombinant M. hyorhinis p37 protein by affinity chromotography. M. hyorhinis p37 (MH38-113) was expressed in E. coli and purified as described previously [16]. Sonicated cell lysate of E. coli was applied to a cobalt affinity column, and the bound protein was eluted with 150 mM imidazole. A total of 25 μg eluate was electrophoresed in a 12% SDS-PAGE gel, and stained with Coomassie blue. M, Prestained BenchMark Protein ladder (kDa); 1, purified recombinant protein. The arrow indicates the position of the recombinant protein.Back to article page