Figure 4
From: Gain of DNA methylation is enhanced in the absence of CTCF at the human retinoblastoma gene promoter
Cell lines with a mutated CTCF binding site are DNA hypermethylated. A, DNA methylation analysis of the wild-type, transcriptionally active Rb core promoter at 100 days of cell culture. B and C, Sodium bisulfite analysis of stable lines 1112 and 2111 after 30 days and 100 days of continuous cell culture were assessed. The asterisks represent the mutated CpGs associated with the CTCF mutation E (CTCFmutE), as defined previously [8].