Figure 4

The ER stress response drives LCN2 transcription in human prostate cancer cells. (A) LNCaP or PC3 cells were treated with Tg (300 nM) for the indicated times. RNA was isolated and analyzed for UPR activation, LCN2, Il-6, and Il-23p19 transcription by RT-qPCR. Data columns indicate the fold difference in transcript level between drug- and vehicle-treated, or untreated cells and error bars represent SEM of 2 biological replicates representative of three independent experiments. (B) LNCaP cells were treated for 18 h with Tg alone, PBA (10 mM) and Tg, or vehicle only. RNA was isolated and analyzed for UPR activation, LCN2, IL-6, and IL-23p19 transcription by RT-qPCR. Data columns indicate the fold difference in transcript level between drug- and vehicle-treated cells and error bars represent SEM of 2-3 biological replicates representative of two independent experiments. Statistical analysis was performed using an unpaired two-tailed t test (***p < 0.001).