In vitro anti-angiogenic properties of LGD1069, a selective retinoid X-receptor agonist through down-regulating Runx2 expression on Human endothelial cells

Table 2 Microvessel Formation of endothelial Cells in vitro (24h)

Group	Total Vessel Actual Perimeter Length (APLT) ± SD(μm)	Formation Rate (%)
CTRL	835.2 ± 91.1	100.00%
LGD1069(7.5 μM)	761.9 ± 59.7	91.22%
LGD1069(15 μM)	601.8 ± 37.4^a	72.05%
LGD1069(30 μM)	109.2 ± 10.6^b	13.07%

HUVECs were seeded in Matrigel-coated 96-well plates, and untreated or treated with LGD1069, in the presence of 2 ng/ml VEGF. Vehicle (DMSO) was utilized as a control. Five fields were counted for each well, and each concentration repeated three wells. Data were expressed as mean ± SD. a p < 0.05, b p < 0.01 compared with control (n = 3).

ISSN: 1471-2407