Figure 7
Suppression of p-Smad2/3 phosphorylation and Runx2 expression in human endothelial cells after incubation with LGD1069 for 24 h, in the presence of 2 ng/ml VEGF. (A) Western blot analysis of protein lysates. (B) densitometric analysis of electrophoretic bands. Intensities were normalized to a β-actin signal. Line 1: Control; Line 2: LGD1069 7.5 μM; Line 3: LGD1069 15 μM; Line 4: LGD1069 30 μM. Vehicle (DMSO) was utilized as a control. Data were expressed as mean ± SD; b p < 0.01 compared with control (n = 3).