Figure 2
Reconstitution of human cord blood derived T lymphocytes in NOD/SCID mice. Cord blood mononuclear cells (1 × 107 cells) were injected into the peritoneum of newborn NOD/SCID mice. A, representative flow cytometry data is shown in indicated hematopoietic organs from NOD/SCID mice injected with cord blood mononuclear cells at 6 weeks after transplantation. B, cells from cord blood or spleen of cord blood injected NOD/SCID mice were compared the expression of CD38 and CD62L expression in CD3+ cells. C, splenocytes from cord blood injected NOD/SCID mice were gated on CD3+ splenocytes, and analyzed for TCRαβ expression. D, immunohistochemical analysis of the thymus (left) and spleen (right) was performed using antibodies to CD4 (FITC) and CD8 (PE). Hoechst dye was used for counterstaining of cell nuclei.