Figure 1From: SIP1 is downregulated in hepatocellular carcinoma by promoter hypermethylation Expression of SIP1 in HCC cell lines and tumors. (A) SIP1 expression is detected in 14 HCC cell lines by multiplex semi-quantitative RT-PCR. GAPDH is used as an internal control. Amplicons corresponding to SIP1 and GAPDH are 132 bp and 611 bp, respectively. A negative control without a DNA template is included. Bands lower than 100 bp in most of the cell lines and negative control sample are primer dimers. M: marker. (B) SIP1 mRNA expression in HCC tumors relative to tumor-adjacent and normal tissues is analyzed by quantitative RT-PCR. Relative mRNA levels of SIP1 normalized to TBP are represented. Patients are grouped depending on tumor stage (GI-GIV). Clinicopathological characteristics of patients (C9-E9) are given in Additional file 1. Standard deviations of two independent experiments are shown.Back to article page