Table 1 Primer and probe sequences
From: Aberrant methylation of N-methyl-D-aspartate receptor type 2B (NMDAR2B) in non-small cell carcinoma
Primer | Assay | Sequence |
|---|---|---|
NMDAR2B | RT-PCR | 5'-GCCTGAGCGACAAAAAGTTC-3' (forward) |
NMDAR2B | RT-PCR | 5'-CATCTCCCCATCTCCAAAGA-3' (reverse) |
GAPDH | RT-PCR | 5'-CACTGGCGTCTTCACCACCATG-3' (forward) |
GAPDH | RT-PCR | 5'-GCTTCACCACCTTCTTGATGTCA-3' (reverse) |
NMDAR2B TAQF | Taqman-MSP | 5'-GAGTATGGTTATTTTTAAAGCG-3' |
NMDAR2B TAQR | Taqman-MSP | 5'-TTAAAACGAATTAATATCTTTTTCG-3' |
NMDAR2B probe | Taqman-MSP | 6FAM5'-ATTCGCGTGTTTTTCGGAGGGTGA-3' TAMRA |
β-actin TAQF | Taqman-MSP | 5'-TGGTGATGGAGGAGGTTTAGTAGTAAGT-3' |
β-actin TAQR | Taqman-MSP | 5'-AACCAATAAAACCTACTCCTCCCTTAA-3' |
β-actin probe | Taqman-MSP | 6FAM5'-ACCACCACCCAACACACAATAACAAACACA-3' TAMRA |