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Table 1 Primer and probe sequences

From: Aberrant methylation of N-methyl-D-aspartate receptor type 2B (NMDAR2B) in non-small cell carcinoma

Primer Assay Sequence
NMDAR2B RT-PCR 5'-GCCTGAGCGACAAAAAGTTC-3' (forward)
NMDAR2B RT-PCR 5'-CATCTCCCCATCTCCAAAGA-3' (reverse)
GAPDH RT-PCR 5'-CACTGGCGTCTTCACCACCATG-3' (forward)
GAPDH RT-PCR 5'-GCTTCACCACCTTCTTGATGTCA-3' (reverse)
NMDAR2B TAQF Taqman-MSP 5'-GAGTATGGTTATTTTTAAAGCG-3'
NMDAR2B TAQR Taqman-MSP 5'-TTAAAACGAATTAATATCTTTTTCG-3'
NMDAR2B probe Taqman-MSP 6FAM5'-ATTCGCGTGTTTTTCGGAGGGTGA-3' TAMRA
β-actin TAQF Taqman-MSP 5'-TGGTGATGGAGGAGGTTTAGTAGTAAGT-3'
β-actin TAQR Taqman-MSP 5'-AACCAATAAAACCTACTCCTCCCTTAA-3'
β-actin probe Taqman-MSP 6FAM5'-ACCACCACCCAACACACAATAACAAACACA-3' TAMRA