Expression and methylation of NMDAR2B in NSCLCs cell lines. Representative examples of reverse transcriptase-polymerase chain reaction results for N-methyl-D-aspartate receptor type 2B (NMDAR2B) expression in lung cancer cell lines (A), and the effect of 5-aza-2'-deoxycytidine on NMDAR2B-negative cell lines (B). Treatment with 5-Aza-CdR restored the expression of NMDAR2B in four cell lines. Expression of the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was run as a control for RNA integrity. M indicates size marker; NC, negative control (water blank); -, before 5-Aza-CdR treatment; +, after 5-Aza-CdR treatment.