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Table 3 Comparison of mean (standard deviation) Ct of reference genes analyzed by RT-qPCR, for the 4 centres

From: Importance of pre-analytical steps for transcriptome and RT-qPCR analyses in the context of the phase II randomised multicentre trial REMAGUS02 of neoadjuvant chemotherapy in breast cancer patients

Reference Gene Centre All centres
  1 2 3 4  
TFR 23.85 (0.87) 24.09 (1.28) 24.23 (1.01) 26.30 (1.82) 24.34 (1.47)
β-actin 17.15 (0.58) 17.34 (1.19) 17.10 (0.62) 18.32 (0.88) 17.38 (0.94)
TBP 26.46 (0.53) 26.53 (1.18) 26.81 (0.47) 28.11 (1.10) 26.77 (1.03)
GAPDH 19.78 (0.94) 19.99 (1.27) 19.90 (0.77) 20.47 (1.05) 19.96 (1.07)
RPLPO 19.44 (0.60) 19.43 (1.06) 19.25 (0.78) 20.51 (0.93) 19.59 (0.91)
GUS 22.84 (0.71) 22.98 (1.23) 23.05 (0.76) 24.83 (1.33) 23.21 (1.21)
p-value referring to ANOVA test for each reference gene, when excluding corresponding centre
TFR < 0.001 < 0.001 < 0.001 0.144 < 0.001
β-actin < 0.001 < 0.001 < 0.001 0.256 < 0.001
TBP < 0.001 < 0.001 < 0.001 0.165 < 0.001
GAPDH 0.075 < 0.001 0.003 0.416 0.006
RPLPO < 0.001 < 0.001 < 0.001 0.562 < 0.001
GUS < 0.001 < 0.001 < 0.001 0.457 < 0.001