Figure 3

Ability of P53 to regulate genes involved in cell cycle is significantly reduced in melanoma. (A) Supervised hierarchical cluster analysis of 728 genes that were significantly regulated by P53 in melanocytes. The relative mRNA expression of these genes in melanocytes, Mel-RM and IgR3 cells that had been stably transduced with either P53 shRNA or control shRNA is shown. (B) Supervised hierarchical cluster analysis of 728 genes that were significantly regulated by P53 in melanocytes. The relative mRNA expression of these genes in melanocytes that had been stably transduced with either P53 shRNA or control shRNA compared to IgR3, Mel-RM, SkMel-28, MM200, Me4405, and Me1007 melanoma cell lines is shown. Genes are coloured according to their expression level, where up-regulated expression is represented by red, down-regulated expression is represented by blue, and equal expression is represented by yellow. (C) The number of genes regulated by P53 (control shRNA versus P53 shRNA) in melanocytes, IgR3 and Mel-RM cell lines in the biological process categories: nucleic acid metabolism, cell cycle, cytokinesis and mitosis as defined by PANTHER [26]. Up-regulated genes are shown in black while down-regulated genes are shown in grey. The number of genes regulated are also depicted as percentages of the total gene list on the bar graph for each of the cell lines. The significance of the regulation of these biological processes by P53 in each of the melanoma cell lines (Mel-RM and IgR3) compared to melanocytes was determined using the gene expression tool in PANTHER (⁺p < 0.0005, ⁺⁺p = 0.000003, *p < 0.05, **p < 0.005).