Figure 7

Chromosome instability in eIF-5A2 transgenic mice. (A) Representative SKY images (left) and reversed DAPI stain (right) of bone marrow metaphase spreads from wild-type and transgenic mice. (B, C) Detection of hallmarks of chromosomal instability in eIF-5A2 transgenic MEFs, including misaligned chromosomes separated from the metaphase plate (a'), lagging chromosomes in anaphase (b' and c'), and micronuclei in interphase cells (C). (D) Quantification of the incidence of the micronuclei, misaligned and lagging chromosomes.