Figure 5

Skeletal aging phenotypes in eIF-5A2 transgenic mice. (A) X-ray radiograph was used to examine skeletal changes in 24-week-old eIF-5A2 transgenic mouse (upper) and their wild-type sibling (lower). Kyphosis was observed in eIF5A2 mouse (indicated by an arrow). (B) Representative radiograph of the density of femur bone in an eIF-5A2 transgenic mouse (24-week-old) and a wild-type mouse. (C, D) Alcian blue and Alizarin red staining of skulls from a 2-week-old transgenic mouse (upper) and its wild-type sibling (lower). A wider cranial sutures (C) and fontanelle (D) were observed in transgenic pup (indicated by arrows). (E) Representative skeletal staining of hind limb in a 2-week-old transgenic mouse (upper) and its wild-type sibling (lower). A clear ossification of patella was observed in the knee joint of wild-type mouse but not in the transgenic mouse (indicated by arrows).