Figure 2

Tissue distribution of transgenic eIF-5A2 protein and BrdU incorporation analysis of day 13.5 embryos. Expression of eIF-5A2 in developing liver, cartilage and neuroepithilium from transgenic and wild-type embryos (left) was detected by IHC (magnification, 400 ×). BrdU incorporation assay (right) was used to compare the proliferation rates between transgenic and wild-type embryos. No significant difference was observed in the percentage of BrdU positive cells between transgenic (56.3 ± 7.81) and wild-type embryos (52.73 ± 9.14, p > 0.05).