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Figure 5 | BMC Cancer

Figure 5

From: Attenuated expression of HRH4 in colorectal carcinomas: a potential influence on tumor growth and progression

Figure 5

HRH4 activation promotes 5-Fu-induced cell apoptosis in colon cancer cells. (A) H4R-Lovo cells were treated with 10mM 5-Fu, 5-Fu together with clozapine(10-5M) or 5-Fu (10mM) and clozapine with JNJ7777120(10-5M) pretreatment, and cell apoptosis was assessed using annexin V/PI staining and flow cytometry. *p < 0.05 vs. control, cells treated with 5-Fu (10mM) alone. (B) H4R-Lovo cells were treated as in Fig.5A, and TUNEL-DAB staining was performed according to the guidance of the kit. *p < 0.05 vs. control, cells treated with 5-Fu alone. (C) H4R-Lovo cells were treated as in Fig.5A, and total cell lysates were examined by Western blotting.

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