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Figure 1 | BMC Cancer

Figure 1

From: Major histocompatibility complex class I-related chain A/B (MICA/B) expression in tumor tissue and serum of pancreatic cancer: Role of uric acid accumulation in gemcitabine-induced MICA/B expression

Figure 1

MICA/B expression in pancreatic cancer cell lines and their sensitivity to NK-92 cell-mediated cytotoxic activity. (A) FACS analysis of MICA/B expression in MRO87 and 7 pancreatic tumor cell lines. Cells (1 × 106/sample) were stained with 1 μg of anti-MCA/B mAb (clone 6D4) followed by 10 μl of fluorescein-conjugated goat-anti-mouse IgG. Isotype-matched mouse IgG was used as a negative control. Cells were analyzed in a BD FACScan flow cytometer. Green line, IgG control; Red line, anti-MICA/B mAb. (B) Cytotoxicity of NK-92 cells against 8 tumor cell lines. Single cell suspensions were prepared and analyzed for cytolysis by NK-92 cells in a standard 51Cr release assay. Experiments were conducted in triplicate and repeated at least twice. The data represent the mean ± standard deviation (SD) from one representative experiment.

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