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Figure 6 | BMC Cancer

Figure 6

From: β-Elemene-induced autophagy protects human gastric cancer cells from undergoing apoptosis

Figure 6

Anti-tumor effect of β-elemene on SGC7901 gastric cancer cells. (A) SGC7901 cells were treated with β-elemene at the indicated concentrations for 24, 48 or 72 h, and the cell viability was analyzed by the MTT assay. Dots: mean of three independent experiments; bars: SD. (B) Cells were treated with 20 or 100 μg/mL β-elemene for 24 h, and cell lysates were subjected to western blotting with specific antibodies. (C) Cells were exposed to either 100 μg/mL β-elemene, 10 mmol/L 3-MA, 20 μmol/L CQ, or a combined treatment of β-elemene and 3-MA or CQ for 24 h, and cell viability was measured by the MTT assay. (D) Cells were treated with 100 μg/mL β-elemene, 10 mmol/L 3-MA, 20 μmol/L CQ, or a combined treatment of β-elemene and 3-MA or CQ for 6 h, and the clone formation ability was analyzed. Columns: mean of three independent experiments; bars: SD. * P<0.05 vs. cells treated with β-elemene alone. (E) Cells were treated as described in A and apoptosis was analyzed by flow cytometry following Annexin V staning.

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