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Figure 1 | BMC Cancer

Figure 1

From: β-Elemene-induced autophagy protects human gastric cancer cells from undergoing apoptosis

Figure 1

Effects of β-elemene on cell viability and apoptosis of MGC803 cells. (A) MGC803 cells were treated with β-elemene at the indicated concentrations for 24, 48 or 72 h, and the cell viability was analyzed by MTT assay. Dots: mean of three independent experiments; bars: SD. (B) MGC803 cells were treated with 10, 50 and 200 μg/mL β-elemene for 24 h, and apoptosis was determined by flow cytometry followed by Annexin V staining. (C) Cells were treated with either 10 or 50 μg/mL β-elemene for 24 h, and the cleavage of caspase 3 and PARP was detected by western blotting. (D) Cells were treated as described above, and the expression of Bcl-2, Bax and Survivin was detected by western blotting. The results were representatives of three independent experiments. Actin was used as loading control. (E) The levels of Bcl-2, Bax and Survivin were measured by NIH Image J software and corrected for actin. Columns: mean of three independent experiments; bars: SD. * P<0.05 vs. untreated control.

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