Figure 6

Coimmunoprecipitation of Stat3/c-met. WT and DN2 cells treated with 100 ng/ml HGF for 30 minutes and untreated controls were immunoprecipitated with total c-met and Stat3 antibodies. Western blot analysis of immunoprecipitates with reciprocal antibodies shows interaction between Stat3 and c-met, which is diminished in DN2 cells. Whole cell lysates probed with total Stat3 and c-met antibodies shown as loading control. IgG controls indicate the use of rabbit IgG instead of antibodies to c-met or Stat3 for the immunoprecipitations.