Figure 5

Expression of tumor markers in WT and DN2 tumors. (A) Paraffin sections of tumors generated from WT and DN2 cells were probed with MMP-2, MMP-9, p-c-met, and total c-met antibodies, followed by an Alexa 546-labeled secondary antibody. Panels were photographed at 600×. (B) Lysates generated from WT and DN2 tumors were resolved on a 4-20% SDS polyacrylamide gel, transferred to nitrocellulose, and probed sequentially with antibodies for p-c-met, total c-met, MMP-2, MMP-9, and β-actin. (C) Bar graph of western blot quantification.