Figure 1

Invasion and scattering of P9WT and S3DN cells in vitro. (A) 25,000 cells cultured in 10% FCS containing media were plated on Matrigel-coated inserts and allowed to invade. Invaded cells were fixed, stained with hematoxylin, and counted. Values represent mean number of cells for triplicate cultures. (B) Quantification of invaded cells for each cell line. Values shown are +/- SEM and P < .05 by Student's T test for WT/S3DN and NEO4/S3DN comparisons indicated by *. (C) Cells growing in 10% FCS-containing media were treated with 100 ng/ml HGF for 24 or 48 hours as indicated and photographed with phase contrast at 100× magnification. (D) Quantification of scattered cells expressed as percent (of total cells) scattered per well. P < .05 by Student's T test for WT/S3DN and NEO4/S3DN comparisons indicated by *.