Figure 3

Loss of MAP2K4 expression affects cellular proliferation. Cellular proliferation was assessed using alamarBlue dye measured by fluorescence at 540/610 nm. Each of three experiments was performed in triplicate and normalized to the 24 h time-point. The average of three biological replicates is shown, except for the 168 h time-point, which was only done twice. Statistical significance was determined by 2-way ANOVA. Error bars are representative of SEM, n = 3.