Figure 2

Aberrant promoter hypermethylation is rare in the MAP2K4 promoter region. A. Map of MAP2K4 promoter region from UCSC Genome browser (Mar. 2006 build) showing the gene in blue (exon 1 indicated by thicker bar), predicted CpG island in green, location of all CpG dinucleotides in orange, and location of the primers and MS-SSCP PCR product in black. MS-SSCP (B) and DNA sequencing electrophoretograms (C) for a representative tumor sample lacking methylation (top) and the positive methylation control SssI treated normal lymphoblast DNA.