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Figure 4 | BMC Cancer

Figure 4

From: Retinoblastoma-independent antiproliferative activity of novel intracellular antibodies against the E7 oncoprotein in HPV 16-positive cells

Figure 4

Mapping of the scFv 43M2 and 51 epitopes on E7 protein by Pep-set™. The HPV16 E7 amino acid sequence from 1 to 54, reproduced as 12-mers with a 9 amino acid overlap, was synthesized on 15 different polypropylene rods. The histogram represents the ability of each 12-mer oligopeptide to bind the purified scFvs as indicated in the figure. Values shown are the averages of those obtained in two different experiments with similar results. The amino acid sequences identified as probable binding epitopes are in bold. The corresponding amino acid sequences for HPV16, HPV18 and HPV33 E7 are aligned in the bottom part of the figure. The conserved residues of HPV18 and 33 in comparison with HPV16 E7 are in bold.

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