Figure 4

Morphological appearance of ARHGDIA knockdown cell lines. Tumor cells were transiently transfected with siRNAs targeting either ARHGDIA (top panels) or non-specific siRNAs (bottom panel, "Neg"). Cells were fixed and fluorescently labeled 72 hours later using primary antibodies to alpha-tubulin ("Tubulin") to show cellular morphology only. Nuclei were visualized using Hoechst stain and classified as either having condensed chromatin indicative of early apoptosis (green color, rightmost panels) and/or aberrantly shaped as a general indicator of cell well-being (blue color, rightmost panels). No obvious differences were seen in the relative numbers of aberrantly shaped nuclei relative to negative control while the numbers of cells containing condensed chromatin were increased in knockdown cells relative to negative control.