Figure 2

Phenotypic analysis of ARHGDIA knockdown cell lines. Tumor cells were transiently transfected with siRNAs targeting either ARHGDIA (top panels) or non-specific siRNAs (bottom panel, "Neg"). Cells were fixed and fluorescently labeled 72 hours later using primary antibodies to alpha-tubulin ("Tubulin") to show cellular morphology only, phospho-histone H3 ("pHH3") to mitosis, or cleaved lamin A to detect apoptosis. Nuclei were visualized using Hoechst stain. No obvious differences were observed in the numbers of pHH3-labeled cells in knockdown cells relative to negative control (based on total cell number) while the number of cleaved lamin A-positive cells were clearly increased in knockdown cells relative to negative control.