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Figure 2 | BMC Cancer

Figure 2

From: Metabolic markers in relation to hypoxia; staining patterns and colocalization of pimonidazole, HIF-1α, CAIX, LDH-5, GLUT-1, MCT1 and MCT4

Figure 2

Immunofluorescent staining pattern of metabolic and hypoxic markers. (a and b) Fluorescent microscopic images of two head and neck carcinomas with different pimonidazole (green) and CAIX (blue) staining patterns. (c - h) Examples of staining of the various markers. (c) LDH-5 (red), pimonidazole (green) and vessels (white). (d) Perinecrotic pimonidazole (green) and CAIX (red) staining, N = Necrosis. (e) MCT1 (red), pimonidazole (green) and vessels (white), note the co-staining of MCT1 with pimonidazole. (f and g) Membranous GLUT-1 (f) and MCT4 (g) (both red) staining in relation to the vessels (white). (h) Nuclear HIF-1α (red) expression in relation to pimoniazole (green) staining. (i and j) Fluorescent microscopic images showing intense HIF-1α (red) staining in a pimonidazole-related (green) CAIX (blue) area and almost absent HIF-1α staining in another CAIX area in the same tissue section.

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BMC Cancer

ISSN: 1471-2407

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