Figure 5

CD248-dependent fibroblast release of trophic factors that regulate tumor cell survival. T241 fibrosarcoma cells (A) and PancO2 pancreatic adenocarcinoma cells (B) were grown in conditioned media (CM) from CD248^WT/WT fibroblasts and CD248^CyD/CyD fibroblasts and cell viability was quantified at 24, 48 and 72 hours as described in Methods. At 48 hours (n = 3) and 72 hours (n = 6), the number of T241 cells exposed to CM from the CD248^CyD/CyD fibroblasts was significantly lower as compared to those exposed to CM from the CD248^WT/WT fibroblasts. No differences in viability were detected with the PancO2 cells. Data represent the mean ± SEM. * p < 0.05; ** p < 0.01.