Loss of aquaporin-4 expression and putative function in non-small cell lung cancer

BMC Cancer

Table 1 Five AQPs were identified to be deregulated (* p-value < 0.05; Fold change ≥ 2 or ≤ 0.5) in tumors versus normal tissues, or in AC versus SCC subtype across different microarray studies

AQP isoform	Microarray Data		qRT-PCR Data		Median Ct value in tissues	Pearson correlation of AQPs
	Tumor vs Normal Fold change	AC vs SCC Fold change	Tumor vs Normal Fold change	AC vs SCC Fold change		AQP1	AQP3	AQP4	AQP5
AQP1	0.18*	7.5*	0.22*	2.36*	27.8	X	x	x	x
AQP3	1.00	4.8*	0.93	5.81*	26.9	0.46	x	x	x
AQP4	0.16*	2.8*	0.11*	5.96*	28.7	0.82	0.42	x	x
AQP5	0.94	6.9*	0.50*	1.88	31.8	0.31	0.40	0.24	x
AQP9	0.26*	0.91	1.05	0.64	31.0	0.39	0.34	0.30	0.10

The results were mainly confirmed by qRT-PCR including information about expression range in all tissues and Pearson correlation between different AQPs.
AQP: aquaporin; AC: adenocarcinoma; SCC: squamous cell carcinoma; fold change was calculated across microarray datasets (median); fold change in bold and marked (*) indicates statistical significance (p-value < 0.05).

ISSN: 1471-2407