(Panel A) Comet assay images of HT29 cells treated with maslinic acid after 24, 48 and 72 h at concentrations of IC
. An increase in the tail moment can be seen after 48 hours' treatment at IC50. (Panel B) Tail-moment values observed in HT29 cells treated with maslinic acid at concentrations of IC50 and IC80 after 24, 48 and 72 h. Percentages compared to untreated control cells are also shown. The values represent means ± SD of four separate experiments. (Panel C) Histograms of cell cycle of HT29 cells after 72 hours' treatment with maslinic acid. Diagram of forward scatter (FS) versus side scatter (SS) of HT29 cells. It can be seen that the central population corresponding to cells in the G0/G1 cell cycle phase was increased. (Panel D) Histograms of cell cycle of HT29 cells after 96 hours' treatment with maslinic acid. A hypodiploid peak can be seen in the sub-G0/G1 region. Diagram of FS versus SS of HT29 cells. Sub-G0/G1 populations are seen to appear at both IC50 and IC80. Values represent means of three separate experiments. Significances are represented as (*) P < 0.05, and (**) P < 0.01.