Effect of constraint PGE
internalisation on cell viability. A: the normalized expression of COX-2, mPGES-1, MRP4 and 15-PGDH transcripts were measured in HCT-116 and SW1116 cells. The graph shown is representative of 2 independent experiments. B: the internalisation of 3H-PGE2 added in the medium in HCT-116Bax-/- cells is shown. C: SW1116 cells were treated with the indicated combination of extracellular PGE2 and inhibitors and cell death was assessed by Trypan blue exclusion. D: HCT-116 and HCT-116Bax-/- cells were treated by 10 μM PGE2 with or without both inhibitors and cell death was assessed by Trypan blue exclusion.