Expression of mPGES-1 and COX-2 in human colon cancer tissues. A: mPGES-1 and COX-2 expression were detected by western blot in 9 human colon cancer protein extracts. Band intensity was quantified and normalized to the corresponding intensity of actin in the sample. B: The expression of mPGES-1 and COX-2 transcripts was measured by RT-PCR in cDNA samples from 7 patients including paired normal and tumour colon tissues and normalized to GAPDH transcript. The significance of the difference between tumour and control tissue is indicated in brackets when the transcript is down-regulated in tumour.