Effects of JP and Gem on PDAC cell apoptosis. AsPC-1 cells were treated with JP (10 μM) and Gem (10 μM) either alone or in combination. (A) The early apoptotic cells (lower right quadrant) were measured by flow cytometry after staining with FITC-conjugated annexin V and propidium iodide. The percentage of cells in each quadrant is indicated within the quadrant. (B) PARP-1 cleavage was measured by Western blotting. The total cell lysate was subjected to SDS-PAGE and immunoblotted with PARP-1 antibody, which recognizes full-length (116 kDa) and cleaved (89 kDa) PARP-1 protein. Expression of α-tubulin (55 kDa) was analyzed as loading control on a separate gel. Data are representative of two independent experiments with similar results.