Table 2 Quantitative methylation-specific PCR primer and TaqMan probe sequences used in the present study
From: Highly frequent promoter methylation and PIK3CA amplification in non-small cell lung cancer (NSCLC)
Genes | Forward primer sequence (5'→3') | Probe sequence (5'→3') | Reverse primer sequence (5'→3') |
|---|---|---|---|
CALCA | GTTTTGGAAGTATGAGGGTGACG | 6FAM-ATTCCGCCAATACACAACAACCAATAAACG-TAMRA | TTCCCGCCGCTATAAATCG |
CDH1 | AATTTTAGGTTAGAGGGTTATCGCGT | 6FAM-CGCCCACCCGACCTCGCAT-TAMRA　 | TCCCCAAAACGAAACTAACGAC　 |
DAPK1 | GGATAGTCGGATCGAGTTAACGTC　 | 6FAM-TTCGGTAATTCGTAGCGGTAGGGTTTGG-TAMRA　 | CCCTCCCAAACGCCGA　 |
EVX2 | TCGTTGGCGGGTGGGTATAG | 6FAM-CTTCACTCCAAACCGCTCCTCATCTCCCG-TAMRA | ACGCCGATAACAACCATTTTAACG |
PAX6 | ATATAGGACGGCGGTTTAGGTTG　 | 6FAM-CCCAAAATCCGACCGACTCCAACCCCTA-TAMRA　 | TTCCGACCGAACGAAAACCTAC　 |
RASSF1A | GCGTTGAAGTCGGGGTTC 　 | 6FAM-ACAAACGCGAACCGAACGAAACCA-TAMRA 　 | CCCGTACTTCGCTAACTTTAAACG 　 |
β-Actin | TGGTGATGGAGGAGGTTTAGTAAGT　 | 6FAM-ACCACCACCCAACACACAATAACAAACACA-TAMRA　 | AACCAATAAAACCTACTCCTCCCTTAA　 |