Table 2 Quantitative methylation-specific PCR primer and TaqMan probe sequences used in the present study
From: Highly frequent promoter methylation and PIK3CA amplification in non-small cell lung cancer (NSCLC)
| Genes | Forward primer sequence (5'→3') | Probe sequence (5'→3') | Reverse primer sequence (5'→3') |
|---|---|---|---|
| CALCA | GTTTTGGAAGTATGAGGGTGACG | 6FAM-ATTCCGCCAATACACAACAACCAATAAACG-TAMRA | TTCCCGCCGCTATAAATCG |
| CDH1 | AATTTTAGGTTAGAGGGTTATCGCGT | 6FAM-CGCCCACCCGACCTCGCAT-TAMRA | TCCCCAAAACGAAACTAACGAC |
| DAPK1 | GGATAGTCGGATCGAGTTAACGTC | 6FAM-TTCGGTAATTCGTAGCGGTAGGGTTTGG-TAMRA | CCCTCCCAAACGCCGA |
| EVX2 | TCGTTGGCGGGTGGGTATAG | 6FAM-CTTCACTCCAAACCGCTCCTCATCTCCCG-TAMRA | ACGCCGATAACAACCATTTTAACG |
| PAX6 | ATATAGGACGGCGGTTTAGGTTG | 6FAM-CCCAAAATCCGACCGACTCCAACCCCTA-TAMRA | TTCCGACCGAACGAAAACCTAC |
| RASSF1A | GCGTTGAAGTCGGGGTTC | 6FAM-ACAAACGCGAACCGAACGAAACCA-TAMRA | CCCGTACTTCGCTAACTTTAAACG |
| β-Actin | TGGTGATGGAGGAGGTTTAGTAAGT | 6FAM-ACCACCACCCAACACACAATAACAAACACA-TAMRA | AACCAATAAAACCTACTCCTCCCTTAA |