Curcumin induces tubulin acetylation. A. DAOY cells were exposed to increasing concentration of curcumin for 3 hours and then immunoblotted with antibodies indicated. Increased acetylation of tubulin was also observed in interphase (B) and mitotic (C) cells after 16 hours of curcumin treatment. Methanol-fixed cells were immunostained with antibody specific for acetylated tubulin (green). DNA stained blue. Bar, 20 μm.