Cellular localization of 14-3-3ε according to the Smad4 expression and KRT23 knock-down status of SW480 cells. A) Confocal immunofluorescence images of SW480 cells. The cellular localization of 14-3-3ε was analyzed in Smad4 negative and Smad4 re-expressing SW480 cells. KRT23 knock-down was achieved by stable overexpression of shKRT1010 in Smad4 re-expressing cells. The cells were double stained with antibodies to 14-3-3ε (red) and the cytosolic marker tubulin (green). B) Validation of Keratin 23 knock-down efficiency. The Keratin 23 expression was monitored by quantitative RT-PCR in stably sh-KRT23 overexpressing SW480 cells as indicated. Each condition was measured in triplicates and normalized to B2M.