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Figure 2 | BMC Cancer

Figure 2

From: Keratin 23, a novel DPC4/Smad4 target gene which binds 14-3-3ε

Figure 2

Tandem affinity purification of Keratin 23. Confocal immunofluorescence images of SW480 cells overexpressing KRT23. A) Smad4 re-expressing SW480 cells B) Smad4 negative SW480 cells. KRT23 was visualized by α-Flag M2 antibody. C) FLAG eluates from the tandem affinity purification experiments with TAP-KRT23 were separated on a standard 1D-PAGE and proteins visualized by silver staining. The protein names corresponding to each numbered band given in table 2.

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