Single agent dose-dependent cytotoxicity induced by cisplatin or gemcitabine. Human bladder cancer cells T24 (A,C) and SW780 (B,D) were seeded in 96 well plates and treatment with the indicated cisplatin or gemcitabine concentration started 24 h after seeding and continued for 48 h. Cell viability was assessed with MTT-assay. Each drug concentration was tested in six individual wells. Real time growth curves monitoring was performed with the RT-CES (RTCA) system. The T24 or SW780 cells were seeded into 16-well or 96-well E-Plates, which contain electrodes integrated into the bottom surfaces of each well that measure cell index based on impedance. Cell index correlates with the area of cells attached to the bottom of the plate. Cisplatin (E) or gemcitabine (F) at the indicated concentration was added 24 h after seeding.