Immunohistochemistry of bladder cancer tissue with antibody against TFAP2α. TFAP2α immunoreactivity of muscle invasive bladder cancer tumors. A and D: Negative stain of nucleus and cytoplasm at 5 and 40 fold objective magnification, respectively. B and E: Negative stain of nucleus and positive stain of cytoplasm (5× and 40×, respectively). C and F: Positive stain of nucleus and cytoplasm (5× and 40×, respectively).