Figure 4
From: Oncostatin M promotes STAT3 activation, VEGF production, and invasion in osteosarcoma cell lines
Src and STAT3 are associated with gp130 in human and canine OSA cell lines. Canine (OSA8) or human (SJSA) OSA cell lines were serum starved for two hours then left untreated or stimulated with rhOSM (50 ng/mL) for 15 minutes. Protein lysates were generated and immunoprecipitation performed for gp130. Co-precipitated proteins were separated by SDS-PAGE and Western blotting performed for total Src, total STAT3, gp130, or β-actin.